Myers Ph.D, Allen C. 

 

Myers, Allen 2010


 Current Appointment:       

            Associate Professor of Medicine

            The Johns Hopkins University School of Medicine

            Department of Medicine

            Division of Allergy and Clinical Immunology

 



Joint Appointment, Associate Professor

            The Johns Hopkins University Bloomberg School of Public Health

            Department of Environmental Health Sciences, Physiology Division

            Baltimore, Maryland

 

 

Personal Data:

Business Address:

The Johns Hopkins Asthma and Allergy Center

5501 Hopkins Bayview Circle, Room 1A.62                          

Baltimore, Maryland 21224

 

Phone:  410-550-2007

Fax:   410-550-2527

email: amyers@jhmi.edu

 

 

Education and Training:

 

            1973-1977                   B.S., Biology

                                                Towson University

                                                Towson, Maryland

 

            1984-1989                   Ph.D., Pharmacology

                                                University of Maryland at Baltimore

School of Medicine

                                                Department of Pharmacology and Experimental Therapeutics

                                                Baltimore, Maryland

 

            1989-1990                   Research Postdoctoral Fellow

                                                The Johns Hopkins University

School of Medicine

                                                Department of Medicine

                                                Division of Pulmonary and Critical Care Medicine

                                                Baltimore, Maryland

 

            1990-1991                   Research Postdoctoral Fellow

                                                The Johns Hopkins University

School of Medicine

                                                Department of Medicine

                                                Division of Clinical Immunology 

                                                Baltimore, Maryland

 

 

Professional Experience:

 

            1978-1983                   Research Technician

                                                The Johns Hopkins University

School of Medicine

                                                Department of Neuroscience

                                                Baltimore, Maryland

 

1990-1992                   Adjunct Lecturer

                                                Department of Biology

                                                University of Maryland Baltimore County

                                                Catonsville, Maryland

 

            1991-1992                   Instructor of Medicine

                                                The Johns Hopkins University

School of Medicine

                                                Department of Medicine

                                                Division of Clinical Immunology

                                                Baltimore, Maryland

 

            1992-1998                   Assistant Professor of Medicine

                                                The Johns Hopkins University

School of Medicine

                                                Department of Medicine

                                                Division of Clinical Immunology

                                                Baltimore, Maryland

 

            1998-present              Associate Professor of Medicine

                                                The Johns Hopkins University School of Medicine

                                                Department of Medicine

                                                Division of Clinical Immunology

                                                Baltimore, Maryland

 

            2003-present              Joint Appointment, Associate Professor

                                                The Johns Hopkins University

                                                Bloomberg School of Public Health

                                                Department of Environmental Health Sciences

                                                Physiology Division

                                                Baltimore, Maryland

 

 


Research Activities

 

PUBLICATIONS

Peer-Reviewed Articles:

 

Pan, J, Rohde, HK, Undem, BJ,  Myers, AC.  (2010) Neurotransmitters in airway parasympathetic neurons altered by NT-3 and repeated allergen challenge. American Journal of Respiratory Cell and Molecular Biology 43(4):452-457.

 

Weigand, LA,  Myers, AC.  (2010) Synaptic and membrane properties of parasympathetic ganglionic neurons innervating mouse trachea and bronchi. American Journal of Physiology (Lung Cellular and Molecular Physiology) 298(4):L593-599.

 

Kollarik M, Carr MJ, Ru F, Ring CJA, Hart VJ, Murdock P, Myers AC, Muroi Y, Undem BJ.

(2010) Transgene expression and effective gene silencing in vagal afferent neurons in vivo using recombinant adeno-associated virus vectors. J Physiol. 588:4303-4315.

Guo JP, Brummet ME, Myers AC, Na HJ, Rowland E, Schnaar RL, Zheng T, Zhu Z, Bochner BS. (2010)  Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. American Journal of Respiratory Cell and Molecular Biology (Epub ahead of print).

 

Yeh, X-C, Xie, L, Langdon J, Myers AC, Oh S-Y, Zhu Z, MacDonald S. (2010) The effects of overexpression of histamine releasing factor (HRF) in an inducible transgenic mouse model. PLoS One 5(6):e11077.

 

Yamaji-Kegan K, Su Q, Angelini DJ, Myers AC, Cheadle C,  Johns RA.  (2010) Hypoxia-induced mitogenic factor (HIMF/FIZZ1/RELM!) increases lung inflammation and  activates pulmonary microvascular endothelial cells via IL-4 dependent mechanism. J. Immunol. 185(9):5539-5548.

 

Reinke C, Bevans-Fonti S, Grigoryev DN, Drager LF, Myers AC, Wise RA, Schwartz AR, Mitzner W, Polotsky VY. Chronic intermittent hypoxia induces lung growth in adult mice (2011) American Journal of Physiology (Lung Cellular and Molecular Physiology)  (In Press).

 

Mazzone, S., Reynolds, S., Mori, M., Kollarik, M., Farmer, D.  Myers, AC, Canning, B.J.  (2009) Selective expression of a sodium pump isozyme by cough receptors and its essential role in regulating cough. Journal of Neuroscience, 29(43):13662-13671.

 

Weigand LA, Myers AC, Meeker S, Undem BJ. (2009) Mast cell-cholinergic nerve interaction in mouse airways. J Physiol. 587:3355-3362.

 

Savransky V., Reinke C., Jun J., Bevans-Fonti S., Nanayakkara A., Li J., Myers AC, Torbenson MS, Polotsky VY. (2009)  Chronic intermittent hypoxia and acetaminophen induce synergistic liver injury. Experimental  Physiology  94(2):228-239.

 

Lee HS, Myers AC, Kim J. (2009)   Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis. Am J Respir Crit Care Med. 80:1056-1067.

 


Peer-Reviewed Articles (cont.)

 

Damarla M, Hasan E, Boueiz A, Le A, Pae HH, Montouchet C, Kolb T, Simms T, Myers AC, Kayyali US, Gaestel M, Peng X, Reddy SP, Damico R, Hassoun PM.  (2009)  Mitogen activated protein kinase activated protein kinase 2 regulates actin polymerization and vascular leak in ventilator associated lung injury.  PLoS One.4(2):e4600.

 

Harvey CJ, Thimmulappa RK, Singh A, Blake DJ, Ling G, Wakabayashi N, Fujii J, Myers AC, Biswal S. (2009)  Nrf2-regulated glutathione recycling independent of biosynthesis is critical for cell survival during oxidative stress. Free Radic Biol Med.  46:443-453.

 

Duarte, AG, Terminella, L, Smith, JT, Myers, AC, Campbell, G, Lick, S.  Restoration of cough reflex in lung transplant recipients. (2008) Chest 134:310-316.

 

Kajekar R, and Myers AC. (2008) Calcitonin gene-related peptide affects synaptic and membrane properties of bronchial parasympathetic neurons. Journal of Respiratory Neurophysiology 160:28-36.

 

Yu SY, Myers AC, Kollarik M, Undem BJ. (2007) Mast cell-mediated long-lasting increases in excitability of vagal C-fibers in guinea pig esophagus. American Journal of Physiology (Gastrointestinal and Liver Physiology) 293:G850-856.

Georas SN, Berdyshev E, Hubbard W, Gorshkova IA, Usatyuk PV, Saatian B, Myers AC, Williams MA, Xiao HQ, Liu M, Natarajan V. (2007)  Lysophosphatidic acid is detectable in human bronchoalveolar lavage fluids at baseline and increased after segmental allergen challenge.  Clinical and Experimental Allergy.  37:311-322.

 

Hazari MS, Pan J, Myers AC.  (2007) Nerve growth factor acutely potentiates synaptic transmission in vitro and induces dendritic growth in vivo on adult neurons in airway parasympathetic ganglia.  American Journal of Physiology (Lung Cellular and Molecular Physiology)  292:L992-1001. 

Wu X, Myers AC, Goldstone AC, Togias A and  Sanico AM. (2006)  Localization of nerve growth factor and its receptors in the human nasal mucosa.  Journal of Allergy and Clinical Immunology  118:428-433.

 

Yokoi H, Myers AC, Matsumoto K, Crocker PR, Saito H, Bochner BS.  (2006) Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells.  Allergy 61(6):769-776.

 

Lane AP, Truong-Tran QA, Myers AC,  Bickel CA,  Schleimer RP. (2006) Serum amyloid A, properdin, complement 3, and toll-like receptors are expressed locally in human sinonasal tissue. American J Rhinology  20:117-123.

 

 Chuaychoo B, Hunter DD, Myers AC, Kollarik M, Undem BJ. (2005) Allergen-induced substance P synthesis in large-diameter sensory neurons innervating the lungs.  J. Allergy and  Clinical Immunol. 116(2):325-31.

 

 Kim J. Myers AC, Chen L, Pardoll D, Truong-Tran QA, Lane A, McDyer J, Fortuno L, Schleimer RP. (2005) Constitutive and inducible expression of B7 family of ligands by human airway epithelial cells.  American J Respiratory and Critical Care Medicine.  33(3):280-289.


Peer-Reviewed Articles (cont.)

 

Tolosa JN, Myers AC, Northington F,  Cooper R, Gauda E.  (2005) Ontogeny of retrograde labeled chemoafferent neurons in the newborn rat nodose-petrosal ganglion complex: ex vivo. Neuroscience Letters   284(1-2): 48-53.

 

Myers AC, Goldie R,  Hay DWP. (2005) A novel role for tachykinin neurokinin-3 receptors in regulation of human bronchial ganglia neurons. American Journal of Respiratory and Critical Care Medicine 171(3):212-216.

 

Oda, N., Canelos P.B., Essayan D.M., Plunkett B.A., Myers AC, Huang S.K. (2005) Interleukin-17F induces pulmonary neutrophilia and amplifies antigen-induced allergic response. American Journal of Respiratory and Critical Care Medicine  171(1):12-8.

 

Xu, K.Y., Takimoto, E., Zhang, Q., Rohde, H.K., Myers, AC (2005) Evidence that the H1-H2 domain of a1 subunit of (Na+-K+)-ATPase participates in the regulation of cardiac contraction. FASEB Journal 19(1):53-61.

 

Undem B.J., Chuaychoo B., Lee M.G., Weinreich D., Myers AC, Kollarik M.  (2004)

Subtypes of vagal afferent C-fibres in guinea-pig lungs  Journal of Physiology (London)   556:905-17.

 

Hung C.H., Chen L.C., Zhang Z., Chowdhury B., Lee W.L., Plunkett B., Chen C.H., Myers AC, Huang S.K. (2004) Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein. J. Allergy and  Clinical  Immunology 114(3):664-70.

 

Wang S.Q., Cheng H., Myers AC, Canning B.J., Xu K.Y.  (2004) Positive inotropic effect induced by sequence-specific Na+-K+-ATPase antibody in intact cardiac myocytes.  Annals of the New York Academy of Science.  986:630-632.

 

Kurosawa S., Myers ACChen L., Wang S., Ni J., Plitt J.R., Heller N.M., Bochner BS, Schleimer RP. (2003) Expression of the costimulatory molecule B7-H2 (ICOS-ligand) by human airway epithelial cells. American Journal of Respiratory and Critical Care Medicine. 28(5):563-73.

 

Kajekar, R., Undem, B.J. and Myers, AC (2003) Role of cyclooxygenase activation and prostaglandins in antigen-induced excitability changes of bronchial parasympathetic ganglia neurons. American Journal  of Physiology  (Lung Cellular and Molecular Physiology). 284(4):L581-587.

 

Canning B.J, Anukwu ., Kajekar R,  Myers AC (2002) Endogenous neurokinins facilitate synaptic transmission in guinea pig airway parasympathetic ganglia. American Journal  of Physiology  (Regulatory, Integrative and Comparative Physiology). 283(2):R320-330.

 

Myers AC,  Kajekar, R.,  Undem, B.J. (2002) Allergic inflammation-induced neuropeptide production in rapidly adapting afferent nerves in guinea pig airways. American Journal  of Physiology  (Lung Cellular and Molecular Physiology). 282(4):L775-781.

 

Myers  AC, Bochner BS, Tomaselli G, F, Fedarko N, Hudson SA.,  Rohde H, Huang S-K, Xu KY (2002)  Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+-K+)-ATPase. Biochemical and Biophysical Research Communications. 291(1): 111-115.


Peer-Reviewed Articles (cont.)

 

Kajekar R,  Rohde HK, Myers AC (2001) The integrative membrane properties of human bronchial parasympathetic ganglia neurons. American Journal of Respiratory and Critical Care Medicine. 164:1927-1032.

 

Chen,  L-C, Zhang,Z, Myers, AC, Huang S-K (2001)  Altered pulmonary eosinophilic inflammation in mice deficient for Clara cell secretory protein, CC10. Journal of Immunology 167(6):3025-3028.

 

Myers AC  (2001) Transmission in autonomic ganglia.  Respiration Physiology  125: 99-111.

 

Undem BJ,  Kajekar R, Hunter DD,  Myers AC (2000)  Neural integration and allergic disease. Journal of Allergy and Clinical Immunology. 106: 213-20.

 

Myers AC (2000) Anatomical characteristics of tonic and phasic postganglionic neurons in guinea pig bronchial parasympathetic ganglia.  Journal of Comparative Neurology. 419:439-450.

 

Hunter DD, Myers AC,  Undem BJ (2000) Nerve growth factor-induced phenotypic switch in guinea pig airway sensory neurons.  American Journal of Respiratory and Critical Care Medicine 161:1985-1990.

 

Kajekar R and Myers AC (2000) Effect of bradykinin on membrane properties of guinea pig bronchial parasympathetic ganglion neurons. American Journal  of Physiology  (Lung Cellular and Molecular Physiology) 278: L485-L491.

 

McAlexander MA,  Myers AC  and Undem BJ (1999) Adaptation of guinea-pig airway neurones to mechanical stimulation. Journal of Physiology (London)   521.1: 230-247.

 

Kajekar R, Proud D, Meeker SN, Myers AC, and Undem BJ  (1999) Characterization of vagal afferent subtypes stimulated by bradykinin in guinea pig trachea.  Journal of Pharmacology and Experimental Therapeutics.  289:  682-687.

 

McAlexander, A.M., Myers, A.C.,  and Undem, B.J. (1998)  Inhibition of 5-lipoxygenase diminishes neurally evoked tachykinergic contraction of the guinea pig isolated airway. Journal of Pharmacology and Experimental Therapeutics  285 (2) 602-607.

 

Myers, A.C. (1998) Calcium and potassium currents regulate action potential accommodation and firing frequency in guinea pig bronchial ganglia neurons. American Journal  of Physiology   (Lung Cellular and Molecular Physiology) 275:  L357-364.

 

Myers, A.C., Riccio, M.M., and Undem, B.J.  (1997)  Effects of nedocromil sodium on neurogenic mechanisms in vitro. Journal of Allergy and Clinical Immunology    98: 107-111.

 

Myers, A.C., Undem, B.J.  and Kummer, W.  (1996) Anatomical and electrophysiological comparison of the sensory innervation of bronchial and tracheal parasympathetic ganglion neurons.  Journal of the Autonomic Nervous System   61: 162-168. 

 

Riccio, M.M., Kummer, W., Biglari, B., Myers, A.C. and Undem, B.J. (1996) Interganglionic segregation of distinct vagal afferent fibre phenotypes in guinea-pig airways. Journal of Physiology (London)   496: 521-530.


Peer-Reviewed Articles (cont.)

 

 

Myers, A.C. and  Undem, B.J.  (1996)  Muscarinic receptor regulation of synaptic transmission in airway parasympathetic ganglia. American Journal of Physiology  (Lung Cellular and Molecular Physiology)  270: L630-L636.   

 

Riccio, M.M.,  Myers, A.C., and Undem, B.J.  (1995) Immunomodulation of afferent neurones in guinea-pig isolated airway. Journal of Physiology (London) 491: 499-509.

 

Myers, A.C. and  Undem, B.J.  (1995) Antigen depolarizes guinea pig bronchial parasympathetic ganglion neurons by activation of histamine H1 receptors. American Journal of Physiology  268 (Lung Cellular and Molecular Physiology 12):  L879-884.

 

Undem, B.J.,  Riccio, M.M.,  Weinreich, D., Ellis,  J.L.  and  Myers, A.C.   (1995) Neurophysiology of mast cell-nerve interactions in the airways  International Archives of Allergy  Immunology. 107: 199-201.

 

Myers, A.C. and  Undem, B.J.  (1993) Electrophysiological effects of tachykinins and capsaicin on guinea-pig bronchial parasympathetic ganglion neurones.  Journal of Physiology  (London) 470: 665-679.

 

Undem, B.J., Myers, A.C. and Weinreich, D. (1991) Antigen-induced modulation of autonomic and sensory neurons in vitro. International  Archives of  Allergy and Applied  Immunology  94: 319-324.

 

Myers, A.C. and Undem, B.J.  (1991) Functional interactions between capsaicin-sensitive and cholinergic nerves  in the guinea pig bronchus.  Journal of Pharmacology and Experimental Therapeutics  259: 104-109.

 

Myers, A.C. and Undem, B.J. (1991) Analysis of preganglionic nerve evoked cholinergic

contractions of the guinea pig bronchus Journal of the Autonomic Nervous System  35: 175-184.

 

Myers, A.C., Undem, B.J. and Weinreich, D.  (1991)  Influence of antigen on membrane properties of guinea pig bronchial ganglion neurons. Journal of  Applied Physiology  71(3): 970-976.

 

Myers, A. C., Undem, B.J. and Weinreich, D. (1990) Electrophysiological properties of neurons in guinea pig bronchial parasympathetic ganglia. American Journal of Physiology (Lung Cellular and Molecular Physiology.   259: L403-L409.

 

Undem, B.J., Myers, A.C., Barthlow, H. and Weinreich, D. (1990) Vagal innervation of guinea pig bronchial smooth muscle. Journal of  Applied Physiology  69: 1336-1346.

 

Millington, W.R., Myers, A.C., and  Bierkamper, G.G. (1985) Diisopropylfluorophosphate inhibits choline efflux from the perfused rat hemidiaphragm. European Journal of  Pharmacology 115: 37-44.

 

Eipper, B.A., Myers, A.C. and Mains, R.A. (1985) Peptidyl-glycine a-amidation activity in tissues and serum of the adult rat. Endocrinology  116:2497-2504.

 

Mains, R.A., Myers, A.C., and Eipper, B.A. . (1985) Hormonal, drug, and dietary factors affecting peptidyl-glycine a-amidating monooxygenase activity in various tissues of the adult male rat. Endocrinology  116: 2502-2515.
Invited Reviews

Book Chapters, Monographs:

 

Myers, A.C. (2007) Electrophysiology of airway nerves. In:  Current Protocols in Pharmacology.  Enna S.J, ed.  John Wiley and Sons Inc., Hoboken, NJ.

 

Undem, B.J. and Myers, A.C.   (2001) Cholinergic and noncholinergic parasympathetic control of airway smooth muscle. In: Muscarinic Receptors in Airways Diseases. Zaagsma, J. Meurs, D., and Roffel, A., eds., Birkhauser Verlang, Switzerland.

 

Undem, B.J.,  Kajekar, R., Hunter, D.D.,  Myers,  A.C. (2000)  Neural Integration and Allergic Disease. Journal of Allergy and Clinical  Immunology. 106(5 Suppl):S213-20.

 

Myers, A.C. (2001) Transmission in Autonomic Ganglia. In: Respiration Physiology Special Edition: Innervation of the Airways. Widdicombe, J.J.  and Canning, B.J., eds., Elsevier Sciences, Amersterdam, Netherlands.

 

Undem, B.J. and Myers, A.C.   (2001) Cholinergic and noncholinergic parasympathetic control of airway smooth muscle. In: Muscarinic Receptors in Airways Diseases. Zaagsma, J. Meurs, D., and Roffel, A., eds., Birkhauser Verlang, Switzerland.

 

Undem, B.J. and Myers, A.C.   (2000)  Neural Regulation of the Immune Response.  In: Asthma and Rhinitis Vol 3.,  Holgate, S.T., and Bussey,  W.W., eds., Blackwell Sci. Pub,  Cambridge, MA..

 

Undem, B.J., Kajekar, R., Myers,  A.C. (2000) Modulation of Peripheral Neurotransmission Associated wtih Mast Cell Activation. In: Mast Cells and Basophils in Physiology, Pathophysiology and Host Defense. G. Marone, Ed. Academic Press.

 

Undem, B.J. and Myers, A.C.   (1999)  Cholinergic and Noncholinergic Parasympathetic Control of Airway Smooth Muscle.  In:  The Autonomic Nervous System,  Zaagsma, J. ed., Gordon and Breach Science Publishers,  London.

 

Pederson, K.E., Myers, A.C., and Undem, B.J. (1998) Neuroimmune Interactions in Airway Inflammation. Eds. Holgate, S.T. and Busse, W.W. In: Lung Biolgoy in Health and Disease: Vol. 117: Inflammatory Mechansims of Asthma, Marcel Dekker.

 

Undem, B.J. and Myers, A.C.   (1997)  Neural Regulation of the Immune Response.  In: Asthma and Rhinitis Vol 2.,  Holgate, S.T., and Bussey,  W.W., eds., Blackwell Sci. Pub,  Cambridge, MA.

 

Undem, B.J. and Myers, A.C. (1996)  Autonomic Ganglia.  In:  The Autonomic Nervous System,  Burnstock, G. ed., Gordon and Breach Science Publishers,  London.

 

Undem, B.J. and Myers, A.C.   (1994)  Neural Regulation of the Immune Response.  In: Asthma and Rhinitis Vol 1.,  Holgate, S.T., and Bussey,  W.W., eds., Blackwell Sci. Pub,  Cambridge, MA.

 

Undem, B.J., D. Weinreich, J.L. Ellis, and A.C. Myers.   (1993)  Neuronal Consequences of the AllergicResponse in the Airways. In: ASTHMA: Physiology, Immunopharmacology, and Treatment. Holgate, S.T., ed., London, 275-286.

 


Invited Reviews

Book Chapters, Monographs (cont.):

 

Undem, B.J. and Myers, A.C.   (1992) Neural Regulation of the Immune Response.  In: Asthma and Rhinitis Vol 1.,  Holgate, S.T., and Bussey,  W.W., eds., Blackwell Sci. Pub,  Cambridge, MA.

 

 

Extramural Sponsorship:

Grants

Title: Neurotrophin Regulation of Airway Parasympathetic Nerves

2008-2012 NIH  Research Scientist Award, RO1 HL088608-01

            total direct costs:  $1,250,000; yearly direct costs: $250,000

            55% Effort as Principal Investigator, Dr. Allen C. Myers PI

 

Title: Efficacy of IgE in Mediating Allergic Reaction In Vivo 

2006-2011, NIH, Asthma and Allergy Disease Research Center, P01 AI070345-01

            total direct costs:  $2,929,952; yearly direct costs: $550,000;

            10% Effort as Core Director, Dr. Donald MacGlashan, PI

 

Title: Molecular Determinants of Pulmonary Arterial Hypertension

            2006-2011 NIH SCORR, 5P50HL084946-02

            total direct costs: $5,329,952: yearly direct costs: $980,000

            10% effort as Molecular Pathology Core Director, Dr. Paul M Hassoun, PI

 

Title: Airway  Sensory Neuroexcitability

2006-2011 NIH Research Scientist Award, R01 HL62296-06           

total direct costs: $928,412, yearly direct costs: $179,699

5% effort as co-investigator with Dr. Bradley J. Undem, PI

 

Title:    Angiogenesis in the lung        

2008-2013 NIH Research Scientist Award, R01HL071605-05

Total direct costs: $1,150,000, yearly direct costs:  $230,000

5% effort as co-investigator with Dr. Elizabeth Wagner, PI

 

Title: Mast Cell-Nerve Interactions in Airways

2008-2013, NIH Research Scientist Award, R01HL38095-15

            Total direct costs: $899,038, yearly direct costs: $143,199

            5% effort as co-investigator with Dr. Bradley J. Undem, PI

 

Title: Local calcium signaling in pulmonary arteries

            2009-2013, Research Scientist Award, R01HL071835-05  SHAM  (PI)                    

Total direct costs: $899,038, yearly direct costs: $220,000

            5% effort as co-investigator with Dr. James Sham, PI

 

Title: Targeting Siglec-8/Siglec-F Reduce Allergic Responses In Vitro and In Vivo 

20010-2015 NIH Research Scientist Award, R01 AI072265-01

Total direct costs: $1,250,000, yearly direct costs:  $250,000

5% effort as co-investigator with Dr Bruce Bochner, PI

 

 


EDUCATIONAL ACTIVITIES

 

Teaching:

Clinical Immunology Training                July-August 1993-present

Physiology                                           Sept-Dec. 2003-present

 

Editorial Activities:

Manuscript reviewer:

            1997-present              Journal of Allergy and Clinical Immunology

            1998-present              American Journal of Respiratory and Critical Care Medicine

            1999-present              American Journal of Physiology

            2002-present              Autonomic Neuroscience: Basic and Clinical

2003-present              Clinical and Experimental Allergy

            2003-present              Respiration Physiology and Neurobiology

            2005-present              Brain Research

2006-present              Pulmonary Pharmacology and Therapeutics

2009-present              Archives of Oral Biology

 

 

 

PEER REVIEW

2004-present  National Science Foundation Research Proposals

            2004-present  National Disease Research Interchange Human Organ

                                    Research Applications

            2008-present Kentucky Science and Engineering Foundation

 

 

CLINICAL ACTIVITIES: none

 

 

ORGANIZATIONAL ACTIVITIES

 

Institutional Administrative Appointments:

Director, Johns Hopkins Bayview Histology Core Laboratory, January 1, 2007-present

 

Chair, Johns Hopkins Asthma and Allergy Center Administration Committee, 1999-present

 

Divisional Representative, Johns Hopkins Office of Safety and Environmental Health, 1996-present

 

Member, Johns Hopkins University Institutional Animal Care and Use Committee, 2004-present

 

Member, Division of Allergy and Clinical Immunology Equipment Acquisition Committee, 2000-present

 

 

Professional Societies:

 

American Physiological Society, 2002-present

 

International Society of Autonomic Neurosciences, 2000-present

 

American Thoracic Society,  1991-2002;  2008-present

 

 


RECOGNITION

 

Invited Talks:

1991    Johns Hopkins School of Medicine, Division of Pulmonary and Critical Care Medicine

 

1992    ICI Americas, Wilmington, DE, Department of Pulmonary Pharmacology

 

1995    Asthma:  Mechanisms, Determinants of Severity, and Treatment, Vancouver, Canada,

            Fisons             Pharmaceutical

 

1997    Johns Hopkins School of Medicine, Division of Pulmonary and Critical Care Medicine

 

1999    The American Thoracic Society-American Lung Association International Meeting Symposium, San Diego,  CA

 

2000    Airway modulation by allergic inflammation.  International Society of the Autonomic Nervous          Neurosciences Millenium Congress, London, UK

 

2001    University of West Virginia, Wheeling, West Virginia, Department of Anatomy

 

2005    Johns Hopkins Medicine International Global Access

 

2007    The Johns Hopkins University Bloomberg School of Public Health

            Department of Environmental Health Sciences, Physiology Division

 

2008    American Physiological Society Experimental Biology Meeting Symposium, San Diego, CA

 

2008    North American Cystic Fibrosis Conference, Orlando, FL


Research Interests

Airway nerves

Acute and chronic airway inflammation

Airway remodeling and histopathology

 

Primary Interest

In the lower airways, airway smooth muscle, vasculature, and glands are under constant control by the parasympathetic nervous system.  By controlling the activity of these cell types, parasympathetic nerves play an important role in regulating air flow to the lungs. For over 50 years, we’ve known that increased parasympathetic nerve activity contributes to reversible airway hyperactivity associated with certain pulmonary diseases such as bronchial asthma and emphysema but the cause and cellular mechanism for abnormal parasympathetic tone is still poorly understood. Other laboratories concentrate on airway smooth muscle and prejunctional release of acetylcholine with relatively few studies focused on the parasympathetic ganglionic neurons “upstream” from the nerve-muscle junction. Due to the pivotal role of ganglionic neuron have in regulation of autonomic tone, our past research involved developing a combination of techniques to study how parasympathetic ganglionic neurons function and respond to inflammation.

Using these techniques, we determined the effects of immediate hypersensitivity (allergic) reactions on membrane characteristics of bronchial parasympathetic ganglia neurons and identified the mediator and neurotransmitter receptors associated with regulation of neuronal activity.   We also spent a considerable effort developing techniques for anatomical and functional analysis of human bronchial parasympathetic ganglionic neurons and analysis of these cells is currently being used in our research.

Current Research

Airway Remodeling and Airway Nerves. In chronic allergy and asthma, the airways are remodeled with increased growth or number of infiltrating cells, fibroblasts, smooth muscle, sensory nerves, and glands, all of these are potential sources of neurotrophic factors and all are in contact with parasympathetic nerves. Neurotrophic factors are growth factors that are usually associated with development of the peripheral nervous system but recently we demonstrated that adult neurons continue to express neurotrophin receptors and activation of these receptors can affect neuronal excitability and morphology. Our current research centers on the hypothesis that the neurotrophic factors serve as important neuro-modulatory autacoids that can regulate the physiology and anatomy  and, consequently, the function  of adult parasympathetic neurons.  Results from these studies will shed new light on the complex pathophysiology of airway diseases such as asthma and COPD and may ultimately determine new therapeutic treatments for these complex diseases.

 

Current research in our labs addresses how neutrophic factors such as the neurotrophin, nerve growth factor (NGF), are involved in dysregulation of parasympathetic nerves at the level of the ganglionic neurons in mice and humans.  For example, the neurotrophin, NGF, is increased in asthmatic airways and acutely affects synaptic transmission in airway parasympathetic ganglia: One effect is an increase the amplitude of the synaptic potential to action potential threshold, thus decreasing integration (filtering) by the neurons.  In our studies, we determine how NGF affects nicotinic receptor channels and electrophysiological properties of human and mouse ganglionic neurons in vitro.

 

We also plan to address the hypothesis that chronic NGF exposure changes neuroanatomical and neurochemical features of mouse and human bronchial ganglionic neurons. Using tissue culture (human) or implanted synthetic devices (Elvax and Matrigel in mice) will determine how long-term exposure of NGF affects dendritic growth and, ultimately, synaptic efficacy.  We will also address the hypothesis that neurotrophins regulate the neurotransmitter phenotypes of mouse and human parasympathetic neurons which may, ultimately alters functional (smooth muscle) responses to nerve stimulation.

 

 

 

Histology and Pathology Core

 

Dr. Myers oversees research by and collaborates with many university and external scientists involving determination and analysis of tissue inflammation and remodeling.  Using over twenty years of histology and pathology research, this laboratory developed advanced staining and histological techniques to identify changes is tissue structure evoked by environmental (allergen, ozone, pollutants) and surgical (mechanical disruption, implants, tracing dyes) interventions, and in genetically altered animals.  In addition, Dr. Myers developed and directs a human tissue biobank repository, providing specimens for translational research.

 

 

Laboratory

Allen C. Myers, Ph.D. Associate Professor of Medicine - senior laboratory investigator; Director, Bayview Histology Core

Holly K. Rohde - Senior Laboratory Technician

Simone Conwell, Research Technician

 

 

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